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KMID : 0811720140180010079
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Korean Journal of Physiology & Pharmacology
2014 Volume.18 No. 1 p.79 ~ p.86
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Phosphorylation of Akt Mediates Anti-Inflammatory Activity of 1-p-Coumaroyl β-D-Glucoside Against Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells
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Vo Van Anh
Lee Jae-Won
Kim Ji-Young
Park Jun-Ho
Lee Hee-Jae
Kim Sung-Soo
Kwon Yong-Soo
Chun Wan-Joo
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Abstract
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Hydroxycinnamic acids have been reported to possess numerous pharmacological activities such as antioxidant, anti-inflammatory, and anti-tumor properties. However, the biological activity of 1-p- coumaroyl β-D-glucoside (CG), a glucose ester derivative of p-coumaric acid, has not been clearly examined. The objective of this study is to elucidate the anti-inflammatory action of CG in lipopo-lysaccharide (LPS)-stimulated RAW264.7 macrophage cells. In the present study, CG significantly suppressed LPS-induced excessive production of pro-inflammatory mediators such as nitric oxide (NO) and PGE2 and the protein expression of iNOS and COX-2. CG also inhibited LPS-induced secretion of pro-inflammatory cytokines, IL-1β and TNF-α. In addition, CG significantly suppressed LPS-induced degradation of IκB. To elucidate the underlying mechanism by which CG exerts its anti-inflammatory action, involvement of various signaling pathways were examined. CG exhibited significantly increased Akt phosphorylation in a concentration-dependent manner, although MAPKs such as Erk, JNK, and p38 appeared not to be involved. Furthermore, inhibition of Akt/PI3K signaling pathway with wort-mannin significantly, albeit not completely, abolished CG-induced Akt phosphorylation and anti- inflammatory actions. Taken together, the present study demonstrates that Akt signaling pathway might play a major role in CG-mediated anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells.
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KEYWORD
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1-p-coumaroyl β-D-glucoside (CG), Akt, COX-2, iNOS, Lipopolysaccharide, NF-κB, RAW264.7 cells
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